Fig. 3
From: KIAA1429 impairs anti-tumor immunity via regulation of PD-L1 and CD8+ T cells in colorectal cancer
A: Protein-protein interaction (PPI) network of KIAA1429 with RNA-related proteins. B: Gene Ontology (GO) enrichment analysis of KIAA1429-related genes. C: SRAMP database predicts a significant m6A binding site on PD-L1 mRNA; D: KIAA1429 shows a positive correlation with PD-L1 expression in CRC tissues(cBioPortal data, P = 2.275e-03); E, F: Immunohistochemistry validated the positive correlation between KIAA1429 and PD-L1 expression in CRC samples (Spearman correlation analysis, P = 1.017e-07); G: Expression levels of KIAA1429 mRNA in various colorectal cancer cell lines, and KIAA1429 is highly expressed in SW620 cells (P < 0.001); H: siRNA-mediated silencing of KIAA1429 (siKIAA1429-1,−2,−3), with siKIAA1429-2 exhibiting the most effective knockdown(P < 0.01); I: Reduced PD-L1 mRNA expression following KIAA1429 knockdown in SW620 cells (P < 0.01); J: Western blot analysis confirmed downregulated PD-L1 protein expression in SW620 cells upon KIAA1429 silencing (P < 0.01).
