Table 1 Schedule of stressors applied during the 56-day CUS protocol.

From: Suramin protects against chronic stress-induced neurobehavioral deficits via cGAS–STING/NF-κB suppression

 

Day1

Day 2

Day 3

Day 4

Day 5

Day 6

Day 7

Week 1

Food and water deprivation (12 h)

Wet bedding (12 h)

Foot shock (0.6 mA for 1 s)

Physical restraint (10 min)

Cold swim (12 °C, 5 min)

Cage tilt

(tilted at 45֯ angle for 1 h)

Tail pinch (1 min)

Week 2

Physical restraint (10 min)

Food and water deprivation (12 h)

Wet bedding (12 h)

Foot shock (0.6 mA for 1 s)

Tail pinch (1 min)

Cold swim (12 °C, 5 min)

Cage tilt (tilted at 45֯ angle for 1 h)

Week 3

Tail pinch (1 min)

Cage tilt (tilted at 45֯ angle for 1 h)

Food and water deprivation (12 h)

Wet bedding (12 h)

Foot shock (0.6 mA for 1 s)

Physical restraint (10 min)

Cold swim (12 °C, 5 min)

Week 4

Cold swim (12 °C, 5 min)

Tail pinch (1 min)

Cage tilt (tilted at 45֯ angle for 1 h)

Food and water deprivation (12 h)

Wet bedding (12 h)

Foot shock (0.6 mA for 1 s)

Physical restraint (10 min)

Week 5

Physical restraint (10 min)

Cold swim (12 °C, 5 min)

Tail pinch (1 min)

Cage tilt (tilted at 45֯ angle for 1 h)

Food and water deprivation (12 h)

Wet bedding (12 h)

Foot shock (0.6 mA for 1 s)

Week 6

Foot shock (0.6 mA for 1 s)

Physical restraint (10 min)

Cold swim (12 °C, 5 min)

Tail pinch (1 min)

Cage tilt (tilted at 45֯ angle for 1 h)

Food and water deprivation (12 h)

Wet bedding (12 h)

Week 7

Wet bedding (12 h)

Tail pinch (1 min)

Physical restraint (10 min)

Cold swim (12 °C, 5 min)

Foot shock (0.6 mA for 1 s)

Cage tilt (tilted at 45֯ angle for 1 h)

Food and water deprivation (12 h)

Week 8

Cold swim (12 °C, 5 min)

Food and water deprivation (12 h)

Tail pinch (1 min)

Cage Tilt (tilted at 45֯ angle for 1 h)

Wet bedding (12 h)

Foot shock (0.6 mA for 1 s)

Physical restraint (10 min)

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