Fig. 1
Difference analysis, functional analysis and heatmap of key gene expression. (A) the variance analysis volcano map, and the Y axis is the average log2FC. a measure that indicates whether the statistical test is statistically significant; the abscissa is indicated for each fraction-treated group and the positive drug group, and the identified genes are the core genes screened in each treatment group. The orange dots in the figure are the up-regulated genes for which p < 0.05 and |log2fc|≥ 1, the blue dots in the figure are the down-regulated genes for which p < 0.05 and |log2fc|≥ -1, and the other genes that do not meet the up-regulation and down-regulation thresholds are the gray dots in the figure. (B) the GO enrichment of differential genes, the outermost circle is GO term, and the size of the middle point is the gene count value. (C) the changes in gene expression in the core pathway after different fraction treatments. The line chart on the left is a clustering of all core genes, and 8 clusters are determined according to the inflection point. Gene expression and function classification diagram of the pathways associated with the fraction experimental group, blank control group and positive control group, and the violin diagram on the left represents the proportion of gene expression of each component in the cluster. The intermediate heatmap shows the change in gene expression of each component; on the right is a comment on the cluster function. (D) Deep learning and positive control comparison to screen medicinal plants, the relationship between three sets of conditions, one of which represents a set, and the number represents the number of intersecting elements in each region.
