Fig. 3 | Scientific Reports

Fig. 3

From: Single-cell identification of an endothelial cell proximal SPP1+ macrophage population defines the metastatic vascular niche in lymph nodes

Fig. 3

SPP1+ TAMs are derived from AREG+ TAMs. (A) Trajectory analysis of TAM populations from MET. Two-dimensional visualization of pseudotime ordering generated by Monocle3. (B) Cells colored by CytoTRACE scores indicating differentiation potential. (C) Left panel: Genes are clustered based on their temporal expression patterns. Middle panel: Heatmap showing dynamic gene expression patterns along pseudotime progression. Right panel: GOBP (green) and KEGG (yellow) pathway enrichment analysis with pseudotime phase. (D) Temporal expression dynamics of key transcription factors, immune-related genes, and angiogenesis-related genes during TAM transition. Red lines represent TAM1_SPP1 signature genes; blue lines represent TAM3_AREG signature genes.

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