Fig. 8 | Scientific Reports

Fig. 8

From: Vitamin D3 attenuates hepatic inflammation in primary biliary cholangitis by inhibiting TLR4/NF-κB mediated M1 macrophage polarization

Fig. 8The alternative text for this image may have been generated using AI.Fig. 8The alternative text for this image may have been generated using AI.

VitD3 suppressed the differentiation of macrophages to M1 through TLR4/NF-κB signaling pathway. (A,B) The expression of iNOS (red) and TLR4 (green) in livers were examined by Immunofluorescence staining, Scale bar = 50 μm, n = 6; Quantification assessment of iNOS and TLR4 fluorescent expression, n = 6; (C,D) The total proteins of the livers were extracted from liver tissue, and the expression levels of iNOS, TLR4, MyD88, p65, pp65, Iκb and p-Iκb were analyzed by Western blot, n = 3. *p<0.05; **p<0.01; ***p<0.001;****p<0.0001.

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