Fig. 1

MamK filaments as observed by AFM under physiological conditions. (A) An AFM image of the MamK filaments polymerized in the presence of 1 mM ATP. (B) A line profile of the height displacement along the black dashed line in the squared area in panel A. Triangles show the peaks of each MamK monomer comprising the protofilament (C, D). Histograms visualizing the length of the half-pitch (C) and diameters (D) of the double-helical filaments (n = 100). The red lines show the Gaussian fit curves. (E) Schematics of the MamK filament size based on the AFM observations. The AFM image was recorded at a rate of 1 s/frame in a 200 × 200 nm scanning area with a resolution of 200 × 200 pixels.