Fig. 4 | Scientific Reports

Fig. 4

From: LINC01116 promotes nascent protein synthesis and cellular stemness through the miR-432-5p/FKBP7/14 regulatory axis in melanoma

Fig. 4

LINC01116 Modulates FKBP7/14 Expression via miR-432-5p. (A-D) Dual-luciferase reporter assays demonstrating the direct binding of miR-432-5p to the 3′-UTR regions of LINC01116, FKBP7/14. (E) qRT-PCR analysis of LINC01116, miR-432-5p, FKBP7/14 expression in A375 and SK-MEL-28 melanoma cell lines transfected with sh-LINC01116 versus sh-NC. (F) Rescue experiments evaluating the effects of miR-432-5p inhibition (via miR-432-5p inhibitor) on FKBP7/14 expression in sh-LINC01116-transfected cells. Co-transfection with inhibitor negative control (Inh-NC) served as a baseline. (G) WB analysis of FKBP7/14 protein levels.

Data were expressed as the mean ± SD, *P < 0.05,**P < 0.01, ***P < 0.001,****P < 0.0001.

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