Fig. 3 | Scientific Reports

Fig. 3

From: LEM4 interacts with β-catenin and promotes β-catenin-dependent transcription activity to confers ribociclib resistance in breast cancer cells

Fig. 3

Activation of β-catenin signaling pathway in ribociclib-resistant cells. (A) Immunoblotting analysis demonstrating reduced GSK3β phosphorylation and increased β-catenin expression in drug-resistant cells (MCF-7 and T47D). (B) Immunoprecipitation of β-catenin followed by Western blot analysis of β-catenin and ubiquitin in parental and ribociclib-resistant cells, showing decreased ubiquitination of β-catenin in resistant cells despite similar total immunoprecipitated β-catenin levels. (C) Quantification of nuclear β-catenin level relative to the cytoplasmic fraction, showing increased nuclear translocation of β-catenin in drug-resistant cells. (D) Measurement of β-catenin-dependent transcription activity in the nuclear fraction, revealing enhanced β-catenin activity in drug-resistant cells. Data are summary of 3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Back to article page