Fig. 4 | Scientific Reports

Fig. 4

From: LEM4 interacts with β-catenin and promotes β-catenin-dependent transcription activity to confers ribociclib resistance in breast cancer cells

Fig. 4

Interaction between LEM4 and β-catenin and its impact on β-catenin stability. (A) Co-immunoprecipitation (co-IP) assay confirming the physical association between LEM4 and β-catenin in MCF-7 cells. (B) Cycloheximide (CHX) chasing experiment demonstrating slower degradation of β-catenin in drug-resistant cells with high LEM4 expression compared to parental cells, and faster degradation upon LEM4 knockdown. (C) Immunoblotting analysis showing reduced total protein level of β-catenin in drug-resistant cells after LEM4 silencing. (D) RT-qPCR analysis showing unaffected mRNA levels of β-catenin upon LEM4 interference. Data are summary of 3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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