Fig. 5 | Scientific Reports

Fig. 5

From: LEM4 interacts with β-catenin and promotes β-catenin-dependent transcription activity to confers ribociclib resistance in breast cancer cells

Fig. 5

LEM4 knockdown and its effects on β-catenin nuclear translocation and transcriptional activity. (A) Decreased amount of β-catenin in the nuclear pool of drug-resistant cells after LEM4 silencing in MCF-7 and T47D cells. (B) Reduced β-catenin activity in the nuclear fraction of drug-resistant cells upon LEM4 knockdown. (C) Expression levels of cell cycle-related genes (MYC, Cyclin D1, Cyclin E) and anti-apoptotic gene (Survivin) in ribociclib-resistant cells. LEM4 knockdown significantly decreased the expression of these β-catenin target genes. Data are summary of 3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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