Fig. 1 | Scientific Reports

Fig. 1

From: Compact Calm1 promoter enables AAV mediated neuron-targeted expression in human iPSC-derived brain organoids

Fig. 1

AAV reporter constructs and experimental workflow. (a, b) Schematic of AAV reporter constructs expressing GFP under the control of either the hSyn promoter or the Calm1 promoter, using an identical backbone. (c) Overview of experimental workflow, including iPSC-derived brain organoid generation, AAV transduction and subsequent analysis. ITR = inverted terminal repeat; hSyn = human synapsin 1 promoter. Calm1 = compact promoter derived from the CALM1 gene4. eGFP = enhanced green fluorescent protein. hGH = human growth hormone polyadenylation signal. iPSCs = induced pluripotent stem cells.

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