Table 1 List of primers used for qPCR analysis and cloning procedures.
Primer | Primer sequence | Orientation | Description |
|---|---|---|---|
1 | gccgcacgcgtgtgtctagactgcagagggccctgcgtatgcgctctgcgcgaggc | forward | PCR primer for gBlock amplification, introducing MluI (5’) restriction site |
2 | ccatggtggcggatccagt | reverse | PCR primer for gBlock amplification, introducing BamHI (3’) restriction site |
3 | catggttccaatgctgtaggtcg | forward | NEUN (RBFOX3) qPCR primer |
4 | ggctacgcagcctacagatacg | reverse | NEUN (RBFOX3) qPCR primer |
5 | cgccgaggtgaagttcgagg | forward | GFP qPCR primer |
6 | cggccatgatatagacgttgtgg | reverse | GFP qPCR primer |
7 | atgggcatctcctccactgtag | forward | EIF4A2 qPCR primer |
8 | cgggattgatgtgcaacaagtg | reverse | EIF4A2 qPCR primer |
