Fig. 4
AppNL-G-F mice show dynamic DNA methylation changes in hippocampus and blood. (A) Venn diagram shows DMR-containing genes shared across different timepoints in blood and hippocampus. (B) Bar heights shows number of DMRs in each timepoint and tissue. Shade within each bar indicates proportion of DMRs that are hypermethylated (darker shade) vs. hypomethylated (white) in AppNL-G-F mice, with exact values also shown. (C) Pie charts show disruption of DMR overlapping various gene features. Intergenic DMRs located > 3 kb from the start or end of a gene were classified as “distal intergenic”. Intergenic DMRs located < 3 kb from a gene start or end were categorized as being “upstream” or “downstream”, respectively. (D) Upset plot shows number of gene associated with at least one DMR across three timepoints and two tissues, illustrating the extent of overlap between these groups. (E) Dot plots display the statistical significance of the top five most enriched biological Gene Ontology (GO) pathways associated with DMRs in the hippocampus and blood.
