Fig. 5 | Scientific Reports

Fig. 5

From: Micro RNA miR-726-3p targets CYB5A in Hen ovaries to modulate granulosa cell proliferation and differentiation

Fig. 5

Overexpression of miR-726-3p in the GCs. (a) The STAR, CYP11A1, CYP17A1 and CYP19A1 mRNA expression in the GCs was analyzed using RT-qPCR. (b, c) Expression of STAR, CYP11A1, CYP17A1 and CYP19A1 proteins in the GCs with or without interference using the overexpression of miR-726-3p was analyzed by western blotting. (d) The PCNA, BCL2 and CASP3 mRNA expression in the GCs was analyzed using RT-qPCR. (e, f) Expression of PCNA, BCL2 and CASP3 proteins in the GCs with or without interference using the Overexpression of miR-726-3p was analyzed by western blotting.

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