Fig. 4 | Scientific Reports

Fig. 4

From: ABCB4 disease-causing variants S242R, S346I, T437I and T1077M significantly impair its function and display differential sensitivity to potentiators

Fig. 4

(A) Representative Western blot analysis of wt and mutants ABCB4. The expression and the processing of ABCB4-wt and the mutants was examined by Western analysis of whole-cell lysates from stably transfected HEK293 cells. ABCB4 expression was detected following SDS-PAGE and immunoblotting with the P3-II-26 antibody. Tubulin was used as a loading control. Molecular masses are indicated on the right (in kDa). Presented data were cropped from full immunoblots shown in supplementary Figure S3. (B) Densitometric quantification of protein amount was performed with iBright Analysis Software. The mature and immature bands were separately quantified on gels, and their relative amounts were calculated. Results are means (± SD) of at least three independent experiments.

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