Table 3 ANOVA test results for retention time (a), number of theoretical plates (NTP, b) and tailing factor (Symmetry) (TF, c) using CCD for various responses.

From: Eco-conscious AQbD-guided stability-indicating RP-UPLC method for analysing Tovorafenib in bulk and dosage forms

Source

Sum of squares

Df

Mean square

F-value

p-value

Result

Model

0.3450

9

0.0383

488.98

< 0.0001

Significant

A-FR

0.2064

1

0.2064

2632.81

< 0.0001

 

B-MP

0.0976

1

0.0976

1245.25

< 0.0001

 

C-TF

0.0215

1

0.0215

274.62

< 0.0001

 

AB

0.0000

1

0.0000

0.2695

0.6150

 

AC

0.0008

1

0.0008

9.95

0.0103

 

BC

0.0006

1

0.0006

8.04

0.0177

 

A²

0.0012

1

0.0012

15.09

0.0030

 

B²

0.0175

1

0.0175

223.50

< 0.0001

 

C²

0.0005

1

0.0005

5.97

0.0346

 

Residual

0.0008

10

0.0001

   

Lack of Fit

0.0005

5

0.0001

1.94

0.2426

Non-significant

Pure Error

0.0003

5

0.0001

   

Cor Total

0.3458

19

    

(a)

Source

Sum of Squares

Df

Mean Square

F-value

p-value

Result

Model

2.566E + 06

6

4.276E + 05

691.31

< 0.0001

Significant

A-FR

9.144E + 05

1

9.144E + 05

1478.42

< 0.0001

 

B-MP

1.395E + 06

1

1.395E + 06

2254.92

< 0.0001

 

C-TF

2.194E + 05

1

2.194E + 05

354.69

< 0.0001

 

AB

9065.31

1

9065.31

14.66

0.0021

 

AC

13292.65

1

13292.65

21.49

0.0005

 

BC

14646.16

1

14646.16

23.68

0.0003

 

A²

8040.71

13

618.52

   

B²

5802.71

8

725.34

1.62

0.3085

Non-significant

C²

2238.00

5

447.60

   

Residual

2.574E + 06

19

    

Lack of Fit

2.566E + 06

6

4.276E + 05

691.31

< 0.0001

Significant

Pure Error

9.144E + 05

1

9.144E + 05

1478.42

< 0.0001

 

Cor Total

1.395E + 06

1

1.395E + 06

2254.92

< 0.0001

 

(b)

Source

Sum of Squares

Df

Mean Square

F-value

p-value

Result

Model

0.1322

9

0.0147

151.48

< 0.0001

Significant

A-FR

0.0072

1

0.0072

74.68

< 0.0001

 

B-MP

0.0382

1

0.0382

394.26

< 0.0001

 

C-TF

0.0065

1

0.0065

66.91

< 0.0001

 

AB

0.0000

1

0.0000

0.5154

0.4892

 

AC

0.0040

1

0.0040

41.75

< 0.0001

 

BC

0.0000

1

0.0000

0.5154

0.4892

 

A²

0.0353

1

0.0353

363.82

< 0.0001

 

B²

0.0260

1

0.0260

267.53

< 0.0001

 

C²

0.0076

1

0.0076

78.52

< 0.0001

 

Residual

0.0010

10

0.0001

   

Lack of Fit

0.0007

5

0.0001

2.42

0.1767

Non-significant

Pure Error

0.0003

5

0.0001

   

Cor Total

0.1332

19

    

(c)

  1. A, Band C coded levels of independent variables; AB, AC and BC are interaction terms; A2, B2 and C2 quadratic terms
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