Table 2 Primer arrangements for qPCR.

From: Novel organoid-based exploration reveals the role of LMTK3/FADS2 signaling in metastatic breast cancer progression in felines and humans

 

Forward

Reverse

Cat

 Cat GAPDH

CTCATGACCACAGTCCATGC

TGAGCTTCCCATTCAGCTCT

 Cat CD44

CGGAAACCTCAAATTCCAGA

TTGTTCACCAAGTGCACCAT

 Cat SOX2

TTTTGTCGGAGACGGAGAAG

GCCGGGCAGTGTGTACTTAT

 Cat YAP1

CTTGTCCGTTGCTGCTGTTA

GCCAGTTGTGGTTTTCCACT

 Cat Bcl2

CCAAGAGGGAAACACCAGAA

GGCCCCGAAAGAAAGAATAG

 Cat Bax

TTTGCTTCAGGGTTTCATCC

GTCCAGTTCATCTCCGATGC

 Cat cyclin D1

CACACGGACTACAGGGGAGT

TCGCAGCATAAGAGTTGGTG

 Cat Cyclin E1

TGCTGAAGATGCACACAACA

CTGGTGTGGTCACACTGTCC

 Cat p21

GCGATGGAACTTCGACTTTG

AGAGTGCAGGACAGGGACAG

 Cat LMTK3

GTGTGGAGACACTGCCCTTT

GGTGAGGTCGGAGGTCAGTA

 Cat FADS2

AGCCCAACATCTTCCACAAG

AGGGCTGTAGGTGAGCAAGA

Human

 Human GAPDH

CGAGATCCCTCCAAAATCAA

TGTGGTCATGAGTCCTTCCA

 Human FADS2

CAGGGTGGAGCTGAGAAGAG

GCCCTTCCCCTGAATCTAAG

 Human LMTK3

CCTACGCTGTGGTCCTCATT

CCCCTTCAGGGTTCTCAAAT

 Human Bax

TTTGCTTCAGGGTTTCATCC

CAGTTGAAGTTGCCGTCAGA

 Human P21

GACACCACTGGAGGGTGACT

GGCGTTTGGAGTGGTAGAAA

 Human cyclin D1

TGTTTGCAAGCAGGACTTTG

TCATCCTGGCAATGTGAGAA

 Human cyclin E1

CCATCCTTCTCCACCAAAGA

TTTGATGCCATCCACAGAAA

Back to article page