Fig. 1
From: Quantitative 3D assessment of muscle dystrophy through X-ray phase-contrast tomography
X-ray phase-contrast tomography (XPCT) images recapitulate the skeletal muscle features of traditional histological analysis. (A) Representative images of transverse or longitudinal sections stained with hematoxylin and eosin and XPCT images of tomographic slices of GA muscles of wild-type (CTR) and dystrophic mice (mdx) at 9 weeks of age. White arrows indicate centrally nucleated myofibers; red arrows indicate degenerating myofibers. Scale bars: 50 μm for XPCT images and 100 μm for cryosections. Quantification of myofiber size by (B) cross-sectional area or (C) minimum Feret’s diameter, and relative variance coefficients, of H&E cryosections (n = 4 muscles per genotype) and XPCT slices (n = 5 muscles per genotype). Results are shown as mean ± sem. *p < 0.05; #p < 0.01; $p < 0.005 by Student’s t-test.
