Figure 2

Identification of NRGs. (A) Multi-group differential expression analysis plots showing differentially expressed genes (DEGs) between stimulated and unstimulated neutrophils across six experimental conditions: A1 (unstimulated 30-minute vs. A23187-stimulated 30-minute), A2 (unstimulated 60-minute vs. A23187-stimulated 60-minute), A3 (A23187-stimulated 30-minute vs. 60-minute), P1 (unstimulated 30-minute vs. PMA-stimulated 30-minute), P2 (unstimulated 60-minute vs. PMA-stimulated 60-minute), and P3 (PMA-stimulated 30-minute vs. 60-minute). (B)Venn diagram illustrating the overlap of the six NRG sets (A1–P3). (C) Bubble plot of GO enrichment analysis for NRGs, including BP, CC, and MF. Dot size and color reflect gene counts and adjusted p-values, respectively. (D) Bubble plot of KEGG pathway enrichment analysis for NRGs. (E)LASSO coefficient profiles of the 670 NRGs.(F) Partial likelihood deviance vs. log(λ) plot for LASSO regression.