Fig. 4
From: Percutaneous extraction using sponge spicules for disease diagnosis and therapeutic drug monitoring
Kinetic study of target compound concentration over time in ISF and blood. (a) Kinetic study of salicylic acid concentration over time in ISF and blood. The real salicylic acid level in blood (red line) and detected salicylic concentration (dark line) from ISF using SHS both increased rapidly after injection and began to decrease half an hour later. They leveled off in an hour. These data showed similar kinetics with no time lag observed for the 30–min time resolution. The predicted salicylic acid level (blue line) in blood from the detected salicylic acid concentration using the formula in Fig. 3b was in good agreement with the real salicylic acid level in blood. After examination, there was no significant discrepancy between the predicted blood salicylic acid level and the measured blood salicylic acid level. **p < 0.01, ns p > 0.05. Mean ± SD of n = 3 independent samples. Two-tailed Student’s t-test. (b) Kinetic study of cyclosporin A concentration over time in ISF and blood. Mean ± SD of n = 3 independent samples. (c) Murine skin grafts with or without cyclosporin A treatment in vivo.
