Table 3 Epididymal sperm plasma membrane functionality, DNA damage, viability, and abnormal morphology.

From: Intratesticular copper oxide nanoparticles induce dose-dependent chemical castration in male mice

Analysis

C

Sham

CuO NPs-5

CuO NPs-10

CuO NPs-20

CuO NPs-40

Sperm plasma membrane (%)

84.04 ± 2.75a

83.53 ± 2.19 ab

80.31 ± 2.12b

76.09 ± 2.29c

62.14 ± 2.69d

33.47 ± 1.13e

Sperm viability (%)

89.53 ± 2.40a

88.39 ± 2.68a

84.43 ± 2.21b

80.31 ± 2.25c

73.93 ± 1.83d

41.60 ± 1.04e

Sperm DNA damage (%)

3.17 ± 0.18e

3.83 ± 0.21e

5.79 ± 0.35d

8.65 ± 0.79c

15.39 ± 1.12b

25.85 ± 1.43a

Sperm abnormal morphology (%)

2.56 ± 0.13e

2.76 ± 0.16e

8.43 ± 0.27d

13.52 ± 0.53c

25.39 ± 1.10b

50.61 ± 1.91a

  1. C: control; CuO NPs-5: 5 mg/mL Copper oxide nanoparticles; CuO NPs-10: 10 mg/mL Copper oxide nanoparticles; CuO NPs-20: 20 mg/mL Copper oxide nanoparticles; CuO NPs-40: 40 mg/mL Copper oxide nanoparticles. a-eDifferent superscripts within the same row demonstrate significant differences (p ≤ 0.05; mean ± SD).