Fig. 14

Protein levels of CCNE1, ULK1, BECN1, CCND1, P27, and P21 in HCC cells analyzed by Western blot. (A) Western blot analysis of CCNE1, ULK1, BECN1, CCND1, P27, and P21 in HepG2 cells transfected with NC or siTOE1 (GAPDH as loading control). (B) Western blot analysis of CCNE1, ULK1, BECN1, CCND1, P27, and P21 in Huh7 cells transfected with NC or siTOE1 (GAPDH as loading control). (C-H) Quantitative analysis of the relative protein levels normalized to GAPDH for each target protein in HepG2 and Huh7 cells. *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant; NC, non-specific control; siTOE1, TOE1 knockdown.