Fig. 4
Heterogeneity of cell annotation and senescence score in single-cell sequencing data of CD patients. (A) Cell annotation marker genes. (B, C) UMAP visualization of the cells were clustered into 22 distinct subclusters and subsequently annotated into 9 major cell types. (D) UMAP visualization of cell types stratified by involved vs. uninvolved regions in CD patients. (E) Pie chart showing cell proportions in involved vs. uninvolved groups (F, G) UMAP plot, single-cell senescence scores based on senescence-associated gene sets and hubgene scores based on hub genes. (H, I) Differential senescence-associated gene scores between involved and uninvolved groups. (J, K) Differential hubgene scores between involved and uninvolved groups. (L) Comparison of senescence scores between involved and uninvolved regions for each individual cell type in CD patients. (M) Comparison of hubgene scores between involved and uninvolved regions for each individual cell type in CD patients. Red indicates higher scores in involved group, blue represents higher scores in uninvolved group, Circle size represents the cell count per population (×1000). *p < 0.05, **p < 0.01, ***p < 0.001.
