Fig. 1

A. Selection protocol to identify potential candidates in the drug repurposing library. The figure shows a summary of the selection protocol that was followed to identify candidates from the re-purposing drug library. B-E: Anti-chlamydial in vitro efficacy of the candidates (bisoprolol fumarate and gemfibrozil, x axis dose series in µM). Relative to positive controls that were untreated and 20% DMSO: Saline controls. C. pecorum and C. muridarum was tested in McCoy B cell culture and % inclusions per field of view assessed. F. in vivo model showing vaginal shedding of infectious chlamydia with intraperitoneal injections of the candidates. Relative to positive controls that were untreated and 20% DMSO: Saline controls. G. Assessment of total infectious yield for statistical significance (Area under the curve (AUC), y axis), and treatment conditions indicated on the x axis.