Table 3 Scoring criteria for mNSS.

From: The role of AIM2 inflammasome pathway-mediated pyroptosis on brain injury induced by severe acute pancreatitis in mice

Category

Score

Scoring criteria

I. Movement Tail Suspension

Tail Suspension

1

Forelimb flexion

 

1

Hindlimb flexion

 

1

Rotation exceeding 10° within 30 s

Placed on Floor (0–3 points)

0

Normal walking

1

Unable to walk in a straight line

2

Circling towards the paralyzed side

3

Falling towards the paralyzed side

II. Sensation Orientation Test

  

1

Orientation test (visual and tactile test)

1

Deep sensation test (placing the paw on the edge of table and pressing the paw to stimulate muscle contraction

III. Balance Beam Test (0–6 points)

  

0

Able to stay stable on the balance beam

 

1

Limbs hanging on the balance beam

 

2

Still hanging on the balance beam, but one limb cannot grip

 

3

Hanging on the balance beam, with two limbs unable to grip or rotating for more than 60 s

 

4

Attempting to balance but falls (more than 40 s)

 

6

Falls: No attempt to balance or grip the balance beam (less than 20 s)

IV. Disappearance of Reflex or Abnormal Activity

  
 

1

Auricle reflex (head shakes when the external auditory canal is stimulated)

 

1

Corneal reflex (blinking when the cornea is stimulated with a cotton swab)

 

1

Startle reflex (reaction when a hard board is tapped quickly with a hand)

 

1

Seizures, myoclonus, dystonia