Fig. 3
Effects of diagnostic ultrasound combined with microbubbles on collagen deposition and angiogenesis in rat wounds. (a) Masson’s trichrome staining under different treatments on days 4, 8, and 12. The USMB group showed the most extensive positive areas (blue-stained regions). Scale bar: 200 μm. (b) Quantitative analysis of blue-stained areas in Masson’s trichrome staining. The USMB group demonstrated the most significant collagen deposition (n = 6). (c) CD31 immunohistochemical staining under different treatments on days 4, 8, and 12. The USMB group exhibited the most abundant positive areas (brown-stained regions). Scale bar: 50 μm. (d) Quantitative analysis of microvessel counting in CD31 immunohistochemistry. The USMB group displayed the highest number of newly formed blood vessels (n = 6). Data are represented as mean with 95% confidence interval. One-way ANOVA with Tukey’s test was performed: **p < 0.01; *p < 0.05; and ns, not significant (p > 0.05). US, ultrasound; USMB, ultrasound combined with microbubbles.
