Fig. 7
miR-26b-5p activates the PI3K/Akt pathway potentially by targeting Gsk3β. (a) Luciferase activity assay in endothelial cells co-transfected with wild-type or mutant Gsk3β 3′UTR-driven luciferase reporters and miR-26b-5p mimic. Cells transfected with the miR-26b-5p mimic exhibited the lowest luciferase activity. (b) qRT-PCR analysis of Gsk3β expression in endothelial cells transfected with miR-26b-5p inhibitor or negative control. Inhibition of miR-26b-5p increased Gsk3β mRNA expression. (c) Western blot analysis of Gsk3β expression in endothelial cells. Transfection with miR-26b-5p inhibitor up-regulated Gsk3β protein levels. (d) Western blot analysis of PI3K/Akt/Gsk3β pathway protein expression in wound tissues from different groups. (e) Quantitative analysis of PI3K/Akt/Gsk3β pathway protein expression in rat wound tissues. The USMB group showed significantly elevated ratios of p-PI3K/PI3K, p-Akt/Akt, and p-Gsk3β/Gsk3β; n = 6. Data are represented as mean with 95% confidence interval. One-way ANOVA with Tukey’s test was performed: **p < 0.01; *p < 0.05; and ns, not significant (p > 0.05). USMB, ultrasound combined with microbubbles.
