Fig. 8
Experimental flowchart. This flowchart illustrates the timeline for establishing the full-thickness skin defect model and the subsequent intervention therapy in rats. On Day 0, the full-thickness skin defect model was established (except for the Control group). Starting from Day 1, intervention therapy was administered every other day, with each session lasting 20 min. The Model group received an injection of normal saline via the tail vein during the treatment; the US group received an injection of normal saline via the tail vein during ultrasonic treatment; the USMB group received an injection of the microbubble suspension via the tail vein during ultrasonic treatment. Photographs were taken and tissue samples were collected on Days 4, 8, and 12. The Control group is not shown in the flowchart, this group did not receive any intervention therapy and was raised under standard conditions.
