Fig. 1
L-lysine administration causes pathological alterations of the mitochondrial ultrastructure in rat pancreatic acinar cells. Isolated rat pancreatic acinar cells were incubated for 2 h with 0–40 mM L-lysine (L-Lys). Cells were then fixed in glutaraldehyde and subjected to dehydration, block staining, embedding and sectioning. An electron microscope was used to acquire images, and the point count method was used to measure the mitochondria/cellular area ratio. Electron microscopic images showing the mitochondria of untreated control cells (a) and the mitochondria of cells after treatment with 10 (b), 20 (c) and (d) 40 mM L-Lys. Arrows point to representative mitochondria. The bar diagram shows the results of the mitochondrial swelling analysis (e). Data are presented as the means ± SEM, parallels were 3 per group and for details please see the supplementary table S1. For statistical analysis, one-way ANOVA was performed followed by Dunnett’s post-hoc test. * indicates statistically significant difference (P < 0.05) compared with the control group. Abbreviations: L-Lys, L-Lysine.
