Fig. 3
L-Lys does not cause pathological elevation of intracellular Ca2+ concentration in pancreatic acinar cells. Isolated mouse acinar cells loaded with the Fura-2-AM fluorescence dye were used to measure the intracellular Ca2+ concentration ([Ca2+]i). Representative F340/F380 trace demonstrates the effect of increasing concentrations (20, 40, 60 mM) of L-Lys (a) on [Ca2+]i. Carbachol (100 µM) was used as the positive control. The effect of treatments with L-Lys and/or BAPTA on ΔΨm was measured after 15 min (b–c), 30 min (d–e), 1 h (f–g), and 2 h (h–i). Representative traces show the relative ΔΨm (normalized to FCCP) of cells at the end of the treatments (b, d, f, h), and raw fluorescent data is presented in supplementary table S7. The average ΔΨm were shown in bar graphs after the treatments (c, e, g, i). Values are mean ± SEM, parallels were 4–11 per group and for details please see the supplementary table S3. Two-way ANOVA was performed in (c, e, g, i) to reveal the effects of L-Lys and/or BAPTA on ΔΨm. This was followed by Šídák’s post-hoc test, and statistically significant differences were detected and marked with * for P < 0.05, ** for P < 0.01, *** for P < 0.001, or **** for P < 0.0001. Abbreviations: +B or -B, with or without BAPTA; +L or -L, with or without L-lysine; L-Lys, L-lysine; norm., normalized.
