Fig. 4
L-lysine treatment of mouse pancreatic acinar cells does not increase trypsinogen expression and activation. (a) After administration of 0 or 40 mM L-Lys to mouse acinar cells over a 2-h time period, cell lysates were subjected to western blot analysis using an anti-trypsinogen and anti-ERK antibodies. The presented images are cropped from two different blots; original full blots with varying exposure times are available in Fig. S3 (Supplementary Figures). (b) Bar graphs show the quantification of the western blot bands of trypsinogen (Tryps) and the loading control ERK. (c) Upon isolation of pancreatic acini, trypsin activity was measured in response to stimulation with or without 40 mM L-Lys at 0.5, 1 and 2 h by a fluorogenic assay using Boc–Gln–Ala–Arg–7-amino-4-methylcoumarin as the substrate. Values are mean ± SEM, parallels were 3 per group and for details please see the supplementary table S4. One-way ANOVA was performed, followed by the Dunnet’s post-hoc test (b), whereas two-way ANOVA was performed, followed by Šídák’s post-hoc test (c). Statistically significant differences are indicated by *P < 0.05.
