Table 1 PGR types and concentrations used in this investigation.
Culture stage | Treatment | PGR in basal MS medium (mg/L) | Other additive (g/L) | |||||
|---|---|---|---|---|---|---|---|---|
2,4-D | NAA | 2iP | BA | GA3 | Agar | AC | ||
Callus initiation | 1 | 10 | – | 3.0 | 6.0 | 1.5 | ||
2 | 50 | 3.0 | 6.0 | |||||
Callus multiplication | 1 | 1.0 | 6.0 | 1.5 | ||||
Embryogenic callus formation | 1 | 0.1 | 6.0 | 1.5 | ||||
Germination & sprouting | 1 | 0.1 | 0.05 | 6.0 | 1.5 | |||
Shoots elongation | 1 | 0.2 | 6.0 | |||||
2 | 0.5 | 6.0 | ||||||
3 | 1.0 | 6.0 | ||||||
Roots formation* | 1 | 0.5 | 1.0 | 1.5 | ||||
2 | 1.0 | 1.0 | 1.5 | |||||
3 | 1.5 | 1.0 | 1.5 | |||||
