Fig. 4

Protein expression profiling using a TMT quantitative proteomics approach. (a) Unsupervised hierarchical clustering analysis of 99 differentially expressed proteins in differentiated SH-SY5Y cells treated with 50 mM BCA (left) and 162 differentially expressed proteins in cells treated with 50 mM GBCA (right) for 48 h. Colour key indicates the log2 expression level. (b) Volcano plots of log2 fold change (x-axis) against log p-value (y-axis) of all 6,827 quantified proteins. Up- and down-regulated proteins are coloured green and red, respectively.