Fig. 4

Single-cell RNA sequencing analysis reveals cellular subpopulation classification in sepsis. (A) Quality control and normalization of scRNA-Seq data from five sepsis samples. (B) Identification of the top 2,000 highly variable genes. (C) Expression levels of MAG-related biomarkers across 22 distinct cell types. (D, E) UMAP and t-SNE plots showing the distribution patterns of the 22 cell types. (F, G) UMAP and t-SNE plots displaying seven major cell types annotated using the SingleR algorithm.