Fig. 3
From: High-throughput isolation of normal and pulmonary fibrosis human alveolar epithelial cells
Analysis for IPF-diseased AT2 cells using the autoMACS Pro Separator (2-step only). (A) Cells/gram of IPF lung tissue after enzymatic and mechanical digestion (n = 11). (B) Purity of AT2 cells (% of EPCAM + /HTII-280 + cells) before and after the 2-step protocol. The purity increased by 81% after cell separation (p < 0.0001). (C) Cell viability (% of live cells/total cells) before and after cell separation. Cellular viability decreased on average by 9% during isolation, from 87 to 78% (p = 0.0016). (D) An average of 2.2 × 105 AT2 cells were obtained per gram of input lung tissue. (E) An example of the flow cytometry analysis for IPF-diseased AT2 cell isolation using the 2-step protocol. 6.2% EPCAM + /HTII-280 + AT2 cells were extracted from the lung tissue by enzymatic and mechanical digestion. 87% of the cells in the EPCAM-/HTII-280- fraction were CD45 positive. CD45 depletion using the autoMACS Pro Separator increased the EPCAM + /HTII-280 + AT2 population to 31.1%. HTII-280-positive enrichment using the autoMACS Pro Separator yielded a final purity of 92% EPCAM + /HTII-280 + AT2 cells. FSC = forward scatter, SSC = side scatter, and the green dashed box indicates AT2 cells. **p < 0.01, ****p < 0.0001 by two-tailed paired t-test.
