Fig. 2
From: Liver injury in a preclinical model of short bowel syndrome is associated with impaired autophagy
p62 (a marker for autophagy) was increased in SBR liver. a. Representative immunohistochemistry slides of p62 in male livers at both 2 and 10 weeks post resection showed increased p62 protein expression in SBR liver (female images are not shown but have similar phenotype). Black arrows represent areas of p62 deposition. b-c. Representative western blots of sham and SBR liver showed increasing protein expression at both 2- and 10-weeks post resection regardless of sex. Both p62 and GAPDH were run on the same gel. Rows were cut and arranged for easier analysis. Unedited blots are in Supplementary Fig. 3a-d. d. rt-qPCR at both 2 and 10 weeks showed elevated p62 mRNA expression in the SBR mice irrespective of sex (2 week—female sham n = 7, female SBR n = 8, male sham n = 10, male SBR n = 5; 10 week—female sham n = 6, female SBR n = 7, male sham n = 7, male SBR n = 8) For panels b, c and d, statistical analysis was completed with unpaired t-test and ANOVA with Tukey correction as appropriate. Bar height represents mean; error bars represent SD. *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.
