Table 1 Formulation and chemical analysis of the experimental diets (% DM basis).

From: Growth, immunity, and antioxidant activity responses of phytobiotics and probiotics incorporated into Oreochromis niloticus diets under stressors of unchanged water

Ingredients g/100 g

Groups

CG

Group 1

Group 2

Group 3

Fish meal

16

16

16

16

Soy bean meal

35.00

35.00

35.00

35.00

Yellow corn

32.80

32.80

32.80

32.80

Wheat bran

12

12

12

12

Starch

1

1

1

1

Soy oil

3

3

3

3

1Vitamin & mineral

0.2

0.2

0.2

0.2

Total

100

100

100

100

Supplementary diets mg/kg

2Garlex®

-

200

400

600

3Superimmune®

-

500

1000

1500

4GallPro-200®

-

200

400

600

Chemical analysis of the basal diet on basis dry matter, %

Dry matter

91.06

Crude protein

29.82

Ether extract

6.8

Crude fiber

4.10

Ash

5.80

5Nitrogen free extract (NFE)

53.48

6 Gross energy kcal/g

4.48

  1. 1-Super mix: Vitamins and minerals mixture each 1Kg of mixture content: 62.5 m I.U. vit A, 25 m I.U. vit D3, 25 g vit E, 1.75 g vit K, 0.5 g vit. B1, 2.75 g vit. B2, 1.25 g vit. B6,10 mg.vit. B12, 20 g.niacin, 500 mg.Folic acid, 50 mg.Biotin, 37 g Zinc, 22 g. Iron, 31 g.Manganese, 2.5 g Copper, 50 mg.Coblat, 113 mg Selenium, 650 mg iodine. Neofarma, Italy.
  2. 2-Garlex Garlic mix, Hebei Kangdali pharmaceutical company, China.
  3. 3-Superimmune® Powder Biological Antimycotoxins And Growth Promoter for Poultry. Composition/kg Purified yeastcell wall extract (Saccharomyces cerevisiae) 980 g, Beta-glucans 240 gm, Mannans 180 g, silicate 10 g and carrier of calcium carbonate up to 1 kg. Neofarma, Italy.
  4. 4- GallPro-200® growth promoter probiotics: Additive/kg.
  5. Bacillus subtillis spore concentrate (DSM 17299), No of spores min.4 × 1012 CFU/kg of product, Sodium aluminon silicate 10 g and carrier of calcium carbonate up to 1 kg. Biochem zusaotzstoffe Handlels-und Produktionsgesellschaft mbH (Küstermeyerstraße, Germany).
  6. 5-NFE was calculated by difference.
  7. 6-Gross energy was calculated according (NRC, 1993).
  8. CG: control was fed a non-supplemented basal diet, at the high stocking (20 fish per 1 m3), and completely unexchanged water with 0% water exchange.
  9. Group 1: fed a basal diet fortified with (200 mg, 500 mg and 200 mg of Garlic mix, Super immune, and Gallipro 200) kg− 1 diet at stocking (20 fish per 1 m3), with 0% water exchange.
  10. Group 2: fed a basal diet fortified with (400 mg, 1000 mg and 400 mg of Garlic mix, Super immune, and Gallipro 200) kg− 1 diet at stocking (20 fish per 1 m3), with 0% water exchange.
  11. Group3: fed a basal diet fortified with (600 mg, 1500 mg and 600 mg of Garlic mix, Super immune, and Gallipro 200) kg− 1 diet at stocking (20 fish per 1 m3), with 0% water exchange.
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