Fig. 3

Generation of Kif26a-deficient mice. (a) Kif26a^−/− mice were generated by the CRISPR/Cas9 system. Exon 7 contains the p-loop ATP binding motif. The arrows represent the PCR primers (see the experimental procedures in Methods). (b–c) Representative results of PCR for genotyping (b) and RT–PCR (c) of the OB. (d–e) In situ hybridization of the OE using kif26a antisense riboprobes in wild-type (d) and kif26a^−/− (e) mice at P0. Scale bars represent 160 μm.