Fig. 6

Increased number of cleaved caspase-3-positive and BrdU-positive cells in the MOE of Kif26a^−/− mice. (a–f) Distribution of cleaved caspase-3 (green) among β-tubulin III (red)-positive cells in the OE of Kif26a^−/− (d–f) and wild-type (a–c) mice at P7. White arrowheads indicate cells positive for both cleaved caspase-3 and β-tubulin III. (g–l) BrdU incorporation assay in the OE at P1, showing BrdU (red)- and β-tubulin III (green)-positive cells in the OE of Kif26a^−/− (j–l) and wild-type (g–i) mice. White arrowheads indicate cells positive for both BrdU and β-tubulin III. The inset shows a high-magnification view of a representative double-labeled cell (l). (m) Quantitative analysis revealed a significant increase in the proportion of cleaved caspase-3-positive apoptotic cells among β-tubulin III-positive cells in Kif26a^−/− mice compared with that in wild-type control mice. (n) Quantitative analysis revealed a significant increase in the proportion of BrdU-positive cells among β-tubulin III-positive cells in Kif26a^−/− mice compared with that in wild-type control mice. The scale bars represent 20 μm.