Fig. 7

Defects in neurite outgrowth in Kif26a^−/− OSNs. (a,b) Representative OSNs dissociated from the olfactory epithelium at P5 and cultured until DIV3 under low-density conditions. Neurons were labeled with an antibody against the neuronal marker anti-β-tubulin III. Compared with shorter Kif26a^−/− OSNs, wild-type OSNs (a) exhibit a bipolar morphology with short, thick dendrites and long, thin axonal processes (b). (c) Quantitative analysis revealed significantly longer axon lengths in cultured wild-type OSNs than in Kif26a^−/− OSNs on DIV3. (d,e) Representative OSNs dissociated from wild-type (d) and Kif26a^−/− (e) olfactory epithelium at P5 and cultured until DIV3 under high-density conditions. (f,g) Representative OSNs dissociated from wild-type (d) and Kif26a^−/− (e) olfactory epithelium at P5 and cultured until DIV10 under high-density conditions. The scale bars represent 50 μm.