Fig. 1

A schematic representation of the subtractive genomics approach: data retrieval, core proteome generation (OrthoFinder), human non-homologous protein filtering (BlastP), subcellular localization (Wolf PSORT), essential gene prediction (Geptop 2.0), druggability analysis (DrugBank + ARG-ANNOT), 3D modeling (SwissModel), validation (PROCHECK), docking (PyRx), and MD simulation (Desmond).