Table 10 The effect of dietary treatment the relative abundance of selected bacterial family in ileal and colonic digesta (mean % relative abundance with their standard errors).

Family	Treatment*				SEM	P value
Grain preservation method	Dried	OA-preserved	Dried	OA-preserved		Grain	ZnO	Grain x ZnO
Zinc oxide supplementation	No	No	Yes	Yes		Grain	ZnO	Grain x ZnO
Ileum
Lactobacillaceae	60.78^ab	56.91^ab	54.53^a	62.68^b	3.080	0.473	0.907	0.050
Lachnospiraceae	9.29^a	2.19^b	5.38^ab	5.30^ab	1.152	0.001	0.393	0.001
Erysipelotrichaceae	0.06	0.51	2.27	1.83	0.569	0.272	0.007	0.179
Eubacteriaceae	0.62	0.46	0.42	0.38	0.299	0.722	0.619	0.872
Oscillospiraceae	0.70	1.72	0.41	0.30	0.536	0.603	0.049	0.282
Ruminococcaceae	7.58	10.99	9.72	12.20	1.483	0.027	0.174	0.571
Enterobacteriaceae	12.53^a	2.08^bc	0.90^b	4.20^c	0.774	0.650	0.002	< 0.001
Clostridiaceae	1.16	2.64	0.89	0.55	0.664	0.663	0.663	0.108
Acidaminococcaceae	0.06	0.09	0.11	0.10	0.131	0.864	0.786	0.840
Hungateiclostridiaceae	1.54	1.34	0.63	0.79	0.473	0.907	0.084	0.647
Colon
Lactobacillaceae	58.96	45.63	65.62	60.03	3.062	0.002	0.001	0.109
Lachnospiraceae	12.80^ab	9.07^a	8.63^a	15.43^b	1.485	0.311	0.555	0.001
Erysipelotrichaceae	0.52	0.20	0.27	1.03	0.383	0.784	0.456	0.092
Eubacteriaceae	1.05	0.47	1.37	1.73	0.498	0.481	0.063	0.207
Ruminococcaceae	13.86	13.55	14.87	14.38	1.503	0.790	0.534	0.959
Clostridiaceae	0.34	0.55	1.01	0.79	0.380	0.815	0.171	0.475
Enterobacteriaceae	0.29^a	5.73^b	1.23^a	1.34^a	0.977	0.002	0.993	0.003

* A total of seven replicates were used per treatment group.
^a,b,c Mean values within a row with unlike superscript letters were significantly different (P < 0.05).

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