Scientific Reports

Table 12 The effect of dietary treatment on the molar proportions and total concentrations of VFA in mmol/gram in pig ileum and colon (Least-square means with their standard errors).

From: Organic acid-preserved grain improves growth and gut health in weanling pigs fed zinc oxide free diets

	Treatment*				SEM	P value
Grain preservation method	Dried	OA-preserved	Dried	OA-preserved		Grain	ZnO	Grain x ZnO
Zinc oxide supplementation	No	No	Yes	Yes		Grain	ZnO	Grain x ZnO
Ileum mmol/gram of digesta
Acetate	0.733	0.787	0.615	0.792	0.0578	0.025	0.244	0.206
Propionate	0.166	0.101	0.255	0.107	0.0447	0.010	0.205	0.268
Butyrate	0.070^ab	0.098^a	0.087^a	0.052^b	0.0129	0.760	0.190	0.009
Valerate	0.008	0.003	0.014	0.014	0.0056	0.604	0.081	0.513
Isobutyrate	0.009	0.003	0.013	0.014	0.0061	0.632	0.152	0.472
Isovalerate	0.014	0.007	0.016	0.019	0.0067	0.787	0.204	0.391
BCFA	0.029	0.022	0.022	0.048	0.0180	0.522	0.538	0.267
Total	12.97	16.67	35.58	20.34	10.099	0.491	0.128	0.264
Colon mmol/gram of digesta
Acetate	0.616	0.588	0.626	0.599	0.0202	0.177	0.602	0.965
Propionate	0.253	0.262	0.221	0.273	0.0133	0.026	0.434	0.101
Butyrate	0.084	0.095	0.093	0.093	0.0108	0.603	0.737	0.633
Valerate	0.016	0.020	0.028	0.013	0.0052	0.270	0.647	0.067
Isobutyrate	0.014	0.016	0.014	0.014	0.0026	0.778	0.742	0.786
Isovalerate	0.017	0.020	0.019	0.018	0.0034	0.674	0.946	0.559
BCFA	0.047	0.056	0.033	0.039	0.0068	0.272	0.029	0.849
Total	151.90	131.22	158.41	144.10	20.346	0.382	0.626	0.872

VFA, volatile fatty acids; BCFA, branched-chain fatty acids. * A total of seven replicates were used per treatment group.

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