Table 4 Primer sequences for QPCR analysis of genes involved gastrointestinal health and function.
Target gene | Gene name | Accession no | Forward primer (5′-3′) Reverse primer (5′-3′) |
|---|---|---|---|
Nutrient transporters | |||
FABP2 | Fatty Acid Binding Protein 2 | NM_001031780.1 | F: CAGCCTCGCAGACGGAACTGAA R: GTGTTCTGGGCTGTGCTCCAAGA |
SLC2A1 | Solute Carrier family 2 Member 1 | XM_003482115.1 | F: TGCTCATCAACCGCAATGA R: GTTCCGCGCAGCTTCTTC |
SLC2A5 | Solute Carrier family 2 Member 5 | EU012359 | F: CCCAGGAGCCGGTCAAG R: TCAGCGTCGCCAAAGCA |
SLC15A1 | Solute Carrier Family 15 Member 1 | NM_214347.1 | F: GGATAGCCTGTACCCCAAGCT R: CATCCTCCACGTGCTTCTTGA |
SLC2A7 | Solute Carrier Family 2 Member 7 | XM_003127552.3 | F: ACATCGCCGGACATTCCATA R: GCGAGGACTGCAGGAAGATC |
SLC5A1 | Solute Carrier Family 5 Member 7 | NM_001164021 | F: GGCTGGACGAAGTATGGTG R: ACAACCACCCAAATCAGAGC |
Short chain fatty acids transporters | |||
SLC16A1 | Monocarboxylate Transporter 1 | NM_001128445.1 | F: GCAGCCCTGTGTTCCTCTCT R: CCAGCCGTAGATACCGAAGAAA |
Response to acid secretion | |||
SCT | Secretin | NM_001009583.1 | F: CCCTGGAGCCTGAAGATTTG R: GCCGGGTTTGAGTTAGCTGAT |
AQP10 | Aquaporin 10 | NM_001128454.1 | F: CTCCGGATCCGCAACCT R: GAGCATGAGCACAAACACACCTA |
GIP | Gastric Inhibitory Polypeptide | NM_001287408.1 | F: AAGAGCGACTGGAAACACAACA R: TTAGACTGATGGGCCAGCTCTAG |
HRH2 | Histamine receptor H2 | XM_003354192.4 | F: CCAGCCTGGATGTCATGCCT R: CCGGTCGAGGCTGATCAT |
APOA4 | Apolipoprotein A-1 | NM_214388.1 | F: GGCTGTGGAACATCTCCAGAA R: GTGTTCACTTCCCCAAGTTTGTC |
Inflammatory markers | |||
IL1A | Interleukin 1A | NM_214029.1 | F: CAGCCAACGGGAAGATTCTG R: ATGGCTTCCAGGTCGTCAT |
IL1B | Interleukin 1B | NM_001005149.1 | F: TTGAATTCGAGTCTGCCCTGT R: CCCAGGAAGACGGGCTTT |
IL6 | Interleukin 6 | NM_214399.1 | F: GACAAAGCCACCACCCCTAA R: CTCGTTCTGTGACTGCAGCTTATC |
CXCL8 | C-X-C motif chemokine ligand 8 | NM_213867.1 | F: TGCACTTACTCTTGCCAGAACTG R: CAAACTGGCTGTTGCCTTCTT |
IL10 | Interleukin 10 | NM_214041.1 | F: GCCTTCGGCCCAGTGAA R: AGAGACCCGGTCAGCAACAA |
IL17 | Interleukin 17 | NM_001005729.1 | F: CCCTGTCACTGCTGCTTCTG R: TCATGATTCCCGCCTTCAC |
IL22 | Interleukin 22 | XM_001926156.1 | F: GATGAGAGAGCGCTGCTACCTGG R: GAAGGACGCCACCTCCTGCATGT |
IFNG | Interferon gamma | NM_213948.1 | F:TCTAACCTAAGAAAGCGGAAGAGAA R: TTGCAGGCAGGATGACAATTA |
TNF | Tumour Necrosis Factor | NM_214022.1 | F: TGGCCCCTTGAGCATCA R: CGGGCTTATCTGAGGTTTGAGA |
FOXP3 | Forkhead box P3 | NM_001128438.1 | F: GTGGTGCAGTCTCTGGAACAAC R: AGGTGGGCCTGCATAGCA |
MMP1 | Matrix Metalloproteinase-histone H3 | NM_001166229.1 | F: GGACCGTGCCATTGAGAAA R: CCTCGGAGACCTTGGTGAAG |
Oxidative stress | |||
NOX1 | NADPH Oxidase 1 | XM_003484140.3 | F: AGCCATGCTGAGATCCCAAT R: TGCTTTATGGCAGGCTTTCA |
Tight junctions | |||
TJP1 | Tight Junction Protein 1 | XM_021098827.1 | F: TGAGAGCCAACCATGTCTTGAA R: CTCAGACCCGGCTCTCTGTCT |
CLDN1 | Claudin 1 | NM 001244539.1 | F: CTGGGAGGTGCCCTACTTTG R: TGGATAGGGCCTTGGTGTTG |
Toll like receptors | |||
TLR2 | Toll-like Receptor 2 | NM_213761.1 | F:CATCTTCGTGCTTTCCGAGAAC R:AAAGAGACGGAAGTGGGAGAAGT |
TLR4 | Toll-like Receptor 4 | NM_001293317.1 | F: TGCATGGAGCTGAATTTCTACAA R: GATAAATCCAGCACCTGCAGTTC |
Mucins | |||
MUC2 | Mucin 2 | AK231524 | F: CAACGGCCTCTCCTTCTCTGT R: GCCACACTGGCCCTTTGT |
Reference genes | |||
HMBS | Hydroxymethylbilane Synthase | NM_001097412.1 | F: CTGAACAAAGGTGCCAAGAACA R: GCCCCGCAGACCAGTTAGT |
H3F3A | Histone H3.3 | NM_213930.1 | F: CATGGCTCGTACAAAGCAGA R: ACCAGGCCTGTAACGATGAG |
YWHAZ | Tyrosine 3-Monooxygenase/tyrtophan 5-monooxygenase Activation Protein Zeta | NM_001315726.1 | F: GGACATCGGATACCCAAGGA R: AAGTTGGAAGGCCGGTTAATTT |
ACTB | Actin Beta | XM_001927228.1 | F:GGACATCGGATACCCAAGGA R:AAGTTGGAAGGCCGGTTAATTT |
B2M | Beta-2-Microglobulin | NM_213978.1 | F:CGGAAAGCCAAATTACCTGAAC R:TCTCCCCGTTTTTCAGCAAAT |
