Table 1 Mesocosm exposure tanks seawater chemistry was taken for the 19-day exposure period and shown as either morning (09:00) or mid-day (12:00) values. Values are ± SD of the mean, and (n) shows the number of independent measurements. pH values are shown in total scale (pHT), and AT represents total alkalinity (µmol kg− 1). pHT and AT were used along with temperature (Temp) and salinity in the R package ‘seacarb’ to produce carbonate chemistry data (HCO3−, CO32−, DIC, pCO2, and Ωarag). Mesocosms were naturally fed seawater from the reef in Kāneʻohe, Hawaiʻi, and to avoid swings in mid-day productivity, the flume environment used seawater from the mesocosms in the morning.
From: Ocean acidification modulates material flux linked with coral calcification and photosynthesis
Time of Day | Treatment | Temp (°C) | Salinity | DO (mg/L) | pHT | AT (µmol kg-1) | HCO3- (µmol kg-1) | CO32- (µmol kg-1) | DIC (µmol kg-1) | pCO2 (µatm) | Ωarag |
|---|---|---|---|---|---|---|---|---|---|---|---|
Holding Mesocosms | |||||||||||
Morning | Control | 26.80 ± 0.18 (n = 6) | 35.52 ± 0.05 (n = 6) | 6.99 ± 0.17 (n = 6) | 7.97 ± 0.03 (n = 6) | 2179 ± 7 (n = 6) | 1714 ± 19 (n = 6) | 187 ± 10 (n = 6) | 1914 ± 11 (n = 6) | 461 ± 33 (n = 6) | 2.99 ± 0.16 (n = 6) |
↑CO2 | 26.83 ± 0.23 (n = 6) | 35.56 ± 0.06 (n = 6) | 6.94 ± 0.12 (n = 6) | 7.67 ± 0.05 (n = 6) | 2178 ± 10 (n = 6) | 1916 ± 24 (n = 6) | 106 ± 11 (n = 6) | 2050 ± 18 (n = 6) | 1030 ± 126 (n = 6) | 1.69 ± 0.18 (n = 6) | |
Mid-day | Control | 27.28 ± 0.29 (n = 30) | 35.39 ± 0.19 (n = 30) | 7.54 ± 0.31 (n = 30) | 8.10 ± 0.04 (n = 30) | 2168 ± 12 (n = 9) | 1584 ± 18 (n = 9) | 234 ± 5 (n = 9) | 1827 ± 15 (n = 9) | 319 ± 11 (n = 9) | 3.75 ± 0.07 (n = 9) |
↑CO2 | 27.25 ± 0.30 (n = 30) | 35.39 ± 0.20 (n = 30) | 7.50 ± 0.23 (n = 30) | 7.83 ± 0.06 (n = 30) | 2174 ± 13 (n = 9) | 1811 ± 24 (n = 9) | 146 ± 10 (n = 9) | 1975 ± 18 (n = 9) | 670 ± 59 (n = 9) | 2.34 ± 0.16 (n = 9) | |
Flume Environment | |||||||||||
Morning | Control | 26.58 ± 0.10 (n = 6) | 35.55 ± 0.04 (n = 6) | 6.86 ± 0.12 (n = 6) | 7.96 ± 0.02 (n = 6) | 2178 ± 10 (n = 6) | 1722 ± 15 (n = 6) | 184 ± 7 (n = 6) | 1918 ± 11 (n = 6) | 470 ± 24 (n = 6) | 2.93 ± 0.11 (n = 6) |
↑CO2 | 26.13 ± 0.23 (n = 6) | 35.61 ± 0.06 (n = 6) | 6.81 ± 0.41 (n = 6) | 7.66 ± 0.03 (n = 6) | 2179 ± 7 (n = 6) | 1927 ± 15 (n = 6) | 102 ± 7 (n = 6) | 2057 ± 12 (n = 6) | 1047 ± 84 (n = 6) | 1.62 ± 0.11 (n = 6) | |
