Fig. 4

Obesity with GDM induces ER stress in the placenta. (A) The ER stress marker CHOP was detected by Immunohistochemistry in the placenta and the quantitative data was shown (n = 12). (B) The ER stress marker pIRE1α was detected by Immunohistochemistry in the placenta and the quantitative data was shown(n = 12). (C) The CHOP protein level was determined by western blotting and the quantitative data was shown (n = 12). (D) The pIRE1α protein level was determined by western blotting and the quantitative data was shown (n = 12). Ctrl: control group. O + G: Obesity with GDM group. Scale bar, 200 μm. Student’s t-test were used to compare the control group with the Obesity with GDM group. ***P<0.001, ****P<0.0001.