Fig. 2
Remimazolam increased cell viability of OGD/R cortical neurons in vitro. (a) The purity of cortical neurons. The cortical neurons were stained for NeuN as neuronal marker (red staining). Nuclei were stained with DAPI. Bar: 20 μm. (b) Neurons were treated with 1, 10, 20, and 50 μM remimazolam. Morphology of cortical neuron. Scale bars: 20 μm. (c) The cell viability of neurons was incubated with different concentrations of remimazolam alone for 12 h by CCK-8. (d) The viability of OGD/R cortical neurons after different concentrations of remimazolam post-treatment. (e) Relative release of LDH in cortical neurons culture medium. All tests were repeated independently three times. Data are presented as Mean ± SEM. ****p < 0.0001: significant as compared to the control group; ##p < 0.01: significant as compared to OGD group.
