Table 2 Comparison of calibration curve parameters for yield of dicentric chromosome aberration in peripheral blood lymphocytes of two healthy donors by using Giemsa or Centromere-FISH staining at 0–1 Gy.

From: Optimized dicentric chromosome dose–response curves with centromere probes improve low-dose radiation biodosimetry

DRC

y = C + αD + βD2

Goodness of fit

Minimum detectable dose

(mGy)

95% CI

(mGy)

C ± SE

α ± SE (p-value)

β ± SE (p-value)

χ2

df

p-value

G2-DRC-1-L

0.0010 ± 0.0003

0.0316 ± 0.0078

(p = 0.0248, p < 0.05)

0.0348 ± 0.0106

(p = 0.0217, p < 0.05)

4.0240

5

0.5640

87

3–233

G2-DRC-2-L

0.0010 ± 0.0004

0.0321 ± 0.0061

(p = 0.0032, p < 0.05)

0.0389 ± 0.0080

(p = 0.0047, p < 0.05)

7.5520

5

0.1827

85

3–226

F2-DRC-1-L

0.0004 ± 0.0002

0.0543 ± 0.0089

(p = 0.0017, p < 0.05)

0.0315 ± 0.0121

(p = 0.0484, p < 0.05)

1.6930

5

0.8898

47

4–142

F2-DRC-2-L

0.0005 ± 0.0002

0.0452 ± 0.0052

(p = 0.0003, p < 0.05)

0.0469 ± 0.0071

(p = 0.0012, p < 0.05)

5.0260

5

0.4127

52

3–157

  1. * Data were derived from two healthy donors (aged 32–38 years; male).
  2. * All dose–response curves (DRCs) were constructed and coefficient and statistical values were obtained from Dose Estimate ver. 5.2.
  3. * Minimum detectable dose and 95% CI were calculated estimated dose by using our fitting curves with Dose Estimate ver. 5.2.
  4. * Dose–response curve conditions were described in Sect. 2.5 of Materials and Methods.
  5. * CI: 95% upper and lower confidence interval of MDD.
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