Fig. 5
The number of mature neurons in the hippocampal dentate gyrus (DG) of TGTR males is higher than in TGNT males. NeuN staining (green) is shown for TGNT (a, n = 7) and TGTR (b, n = 7) males. Images on the right represent positive NeuN signal derived for quantification. Scale bars = 1000 μm. TGTR males had increased NeuN counts/nm2 in the DG hippocampal subregion than TGNT males (c). There was no significant difference in NeuN count/nm2 in the DG region in female rats (d). Ordinary two-way ANOVA with Tukey’s post hoc tests were used for quantification. For simplicity, error bars are shown in one direction only. Hippocampi were also stained for phosphoTau (red, AT8 antibody). There were no significant differences of hyperphosphorylated tau levels in male rats across genotypes and treatments. TGNT—transgenic not treated; TGTR—transgenic BT-11 treated. DG = dentate gyrus; t = treatment effect.
