Fig. 4

Drosophila tolerate dietary Aureobasidium pullulans and Rhodotorula taiwanensis. (A) Schematic of egg laying cups. Harvested R. taiwanensis and A. pullulans were placed on an agar plate for fly consumption (bottom). (B,C) Larva (B) and adult female fly (C) after feeding on R. taiwanensis. Red guts (arrows) indicate the animals ingested the fungi. (D,E) Larva (D) and adult female fly (E) after feeding on irradiated A. pullulans. Guts show the animals ingested the radiation-induced melanized fungi (arrows). (F) The percentage of larvae that pupated (solid lines) and eclosed (dashed lines) after being fed exclusively on alternative fungi. Each point on the graph represents the average of triplicates. Error bars represent standard deviation. (G) Lifespan analysis of males and females exclusively fed A. pullulans or R. taiwanensis. Agar only plates had no corn syrup (pink lines). Graphs were plotted using Microsoft Excel. Each point on the lifespan represents the average of triplicates of twenty flies that were fed fungi in parallel.